When it comes to oceans beneath the surface of icy moons, Europa is the usual suspect. Indeed, Europa Clipper should have much to say about the moon’s inner ocean when it arrives in 2030. But Titan, often examined for the possibility of unusual astrobiology, has an internal ocean too, beneath tens of kilometers of ice crust. The ice protects the mix of water and ammonia thought to be below, but may prove to be an impenetrable barrier for organic materials from the surface that might enrich it.

I’ve recently written about abused terms in astrobiological jargon, and in regards to Titan, the term is ‘Earth-like,’ which trips me every time I run into it. True, this is a place where there is a substance (methane) that can appear in solid, liquid or gaseous form, and so we have rivers and lakes – even clouds – that are reminiscent of our planet. But on Earth the cycle is hydrological, while Titan’s methane mixing with ethane blows up the ‘Earth-like’ description. For methane to play this role, we need surface temperatures averaging −179 °C. Titan is a truly alien place.

In Titan we have both an internal ocean world and a cloudy moon with an atmosphere thick enough to keep day/night variations to less than 2 ℃ (although the 16 Earth day long ‘day’ also stabilizes things). It’s a fascinating mix. We’ve only just begun to probe the prospects of life inside this world, a prospect driven home by new work just published in the Planetary Science Journal. Its analysis of Titan’s ocean as a possible home to biology doesn’t come up completely short, but it offers little hope that anything more than scattered microbes might exist within it.

Image: This composite image shows an infrared view of Titan from NASA’s Cassini spacecraft, acquired during a high-altitude fly-by, 10,000 kilometers above the moon, on Nov. 13, 2015. The view features the parallel, dark, dune-filled regions named Fensal (to the north) and Aztlan (to the south). Credit: NASA/JPL/University of Arizona/University of Idaho.

The international team led by Antonin Affholder (University of Arizona) and Peter Higgins (Harvard University) looks at what makes Titan unique among icy moons, the high concentration of organic material. The idea here is to use bioenergetic modeling to look at Titan’s ocean. Perhaps as deep as 480 kilometers, the ocean can be modeled in terms of available chemicals and ambient conditions, factoring in energy sources (likely from chemical reactions) that could allow life to emerge. Ultimately, the models rely on what we know of the metabolism of Earth organisms, which is where we have to start.

It turns out that abundant organics – complex carbon-based molecules – may not be enough. Here’s Affholder on the matter:

“There has been this sense that because Titan has such abundant organics, there is no shortage of food sources that could sustain life. We point out that not all of these organic molecules may constitute food sources, the ocean is really big, and there’s limited exchange between the ocean and the surface, where all those organics are, so we argue for a more nuanced approach.”

Nuance is good, and one way to explore its particulars is through fermentation. The process, which demands organic molecules but does not require an oxidant, is at the core of the paper. Fermentation converts organic compounds into the energy needed to sustain life, and would seem to be suited for an anaerobic environment like Titan’s. With abiotic organic molecules abundant in Titan’s atmosphere and accumulating at the surface, a biosphere that can feed off this material in the ocean seems feasible. Glycine, the simplest of all known amino acids and an abundant constituent of matter in the early Solar System, serves as a useful proxy, as it is widely found in asteroids and comets and could sink through the icy shell, perhaps as a result of meteorite impacts.

The problem is that this supply source is likely meager. From the paper:

Sustained habitability… requires an ongoing delivery mechanism of organics to Titan’s ocean, through impacts transferring organic material from Titan’s surface or ongoing water–rock interactions from Titan’s core. The surface-to-ocean delivery rate of organics is likely too small to support a globally dense glycine-fermenting biosphere (<1 cell per kg water over the entire ocean). Thus, the prospects of detecting a biosphere at Titan would be limited if this hypothetical biosphere was based on glycine fermentation alone.

Image: This artist’s concept of a lake at the north pole of Saturn’s moon Titan illustrates raised rims and rampart-like features as seen by NASA’s Cassini spacecraft. Credit: NASA/JPL-Caltech.

More biomass may be available in local concentrations, perhaps at the interface of ocean and ice, and the authors acknowledge that other forms of metabolism may add to what glycine fermentation can produce. Thus what we have is a study using glycine as a ‘first-order approach’ to studying the habitability of Titan’s ocean, one which should be followed up by considering other fermentable molecules likely to be in the ocean and working out how they might be delivered and in what quantity. We still wind up with only approximate biomass estimates, but the early numbers are low.

The paper is Affholder et al., “The Viability of Glycine Fermentation in Titan’s Subsurface Ocean,” Planetary Science Journal Vol. 6, No. 4 (7 April 2025), 86 (full text).